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本研究旨在探讨转化生长因子-β2(TGF-β2)在猪骨髓间充质干细胞(pBMMSCs)衰老中的作用及机制。首先分析pBMMSCs在体外复制性衰老过程中TGF-β2水平的变化，研究1 ng/m L、10 ng/mL和100 ng/m L的TGF-β2对细胞生长、增殖、衰老、线粒体活性氧(mtROS)水平、细胞周期及相关基因表达的影响。结果显示，pBMMSCs表达干细胞标志物CD90和CD44，具备成脂和成骨分化潜能。随着传代次数的增加，TGF-β2的mRNA水平显著升高，蛋白质的质量浓度从(41.97±9.46)pg/m L上升到(77.04±3.44)pg/m L。TGF-β2处理后，细胞增殖能力下降，衰老细胞显著增加，mtROS水平升高，细胞周期阻滞在G1期，OCT4、Ki67、PCNA、SOD2表达下降，P53和P21表达上升。上述结果表明，TGF-β2通过促进线粒体活性氧积累并阻滞细胞周期G1期，诱导pBMMSCs衰老。本研究为深入理解TGF-β2在间充质干细胞衰老过程中的作用提供了参考。
Abstract：This study aimed to investigate the role and mechanism of transforming growth factor-beta 2(TGF-β2)in the senescence of porcine bone marrow mesenchymal stem cells(pBMMSCs). First,changes in TGF-β2 levels during in vitro replicative senescence of pBMMSCs were analyzed. Then,the effects of TGF-β2 at concentrations of 1 ng/mL,10 ng/mL,and 100 ng/mL on cell growth,proliferation,senescence,mitochondrial reactive oxygen species(mtROS)levels,cell cycle,and related gene expression were also examined. The results showed that pBMMSCs expressed stem cell markers CD90 and CD44,and had the potential for adipogenic and osteogenic differentiation. With increasing passage numbers,the mRNA levels of TGF-β2 significantly increased,and its protein content rose from(41.97±9.46)pg/m L to(77.04±3.44)pg/mL. After TGF-β2 treatment,cell proliferation ability decreased,the number of senescent cells significantly increased,mtROS levels were elevated,and the cell cycle was arrested at G1 phase. Moreover,the expression of OCT4,Ki67,PCNA,and SOD2 was downregulated,while the expression of P53 and P21 was upregulated. These findings indicated that TGF-β2 induced the aging of pBMMSCs by promoting mtROS accumulation and causing G1-phase cell cycle arrest. This study provides new insights into the role of TGF-β2 in the senescence process of mesenchymal stem cells.
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基本信息:
DOI：10.13364/j.issn.1672-6510.20240250
中图分类号:Q255
引用信息:
[1]马鑫淼,徐德旭,张智霞,等.TGF-β2通过增加线粒体活性氧积累和阻滞细胞周期促进猪间充质干细胞衰老[J].天津科技大学学报,2026,41(03):27-37.DOI:10.13364/j.issn.1672-6510.20240250.
基金信息:
国家重点研发计划项目（2021YFC2101403）
2026-06-25
2026-06-25