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以超声辅助醇提四棱豆叶总酚提取量为考察指标，在单因素实验的基础上，通过响应面实验优化总酚的提取工艺。以DPPH自由基、ABTS+自由基、羟自由基清除率以及亚铁离子与三吡啶基三嗪(FRAP还原力)4种抗氧化能力分析方法，考察四棱豆叶总酚的抗氧化能力。结果表明：四棱豆叶总酚的最佳提取工艺为乙醇体积分数38%、提取温度67℃、料液比(g∶m L)1∶26、超声功率300 W、提取时间40 min，在该条件下四棱豆叶总酚提取量达到6.01 mg/g。四棱豆叶总酚提取物对DPPH自由基、ABTS+自由基、羟自由基具有较强的清除作用，其IC50分别为2.09 mg/m L、0.39 mg/m L、0.000 32 mg/mL。液相色谱-串联质谱(LC-MS/MS)技术分析结果表明，四棱豆叶醇提物中的酚酸类化合物成分共有31种，其中邻羟基肉桂酸对其抗氧化能力贡献较大。
Abstract：In the present study,the extraction of total phenols from the leaves of winged bean was investigated by ultrasound-assisted alcoholic extraction,and the extraction process of total phenols was optimised by response surface test on the basis of one-way test. And the antioxidant capacity of total phenols was investigated by four antioxidant capacity analyses,namely DPPH·,ABTS+,·OH radical scavenging rate,and ferrous ion and tripyridyltriazine(FRAP reducing power). The results showed that the optimal extraction process of total phenol was 38% ethanol volume fraction,extraction temperature of 67 ℃,material-liquid ratio of 1∶26(g/m L),ultrasonic power 300 w,and extraction time of 40 min,and the extracted amount of total phenol reached 6.01 mg/g under these conditions. Moreover,the extract of total phenol had a stronger scavenging effect on the DPPH,ABTS+,and OH free radicals,and its DPPH·,ABTS+,and ·OH with IC50 of 2.09 mg/m L,0.39 mg/m L,and 0.000 32 mg/mL,respectively. Analysis using LC-MS/MS showed that there were 31 phenolic acid components in the alcoholic extracts of winged bean leaves,of which o-hydroxycinnamic acid provided a greater contribution to its antioxidant capacity.
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基本信息:
DOI：10.13364/j.issn.1672-6510.20240057
中图分类号:TS201.2
引用信息:
[1]何洪鑫,孙风霞,王赫等.四棱豆叶总酚提取工艺优化及抗氧化能力研究[J].天津科技大学学报,2025,40(02):29-36.DOI:10.13364/j.issn.1672-6510.20240057.
基金信息:
宁夏回族自治区重点研发计划项目(2022BBF03026)